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目的 为中药黄连寻找简便、适用的含量测定方法。方法 用酸性染料比色法和薄层色谱扫描法测定黄连中小蘖碱的含量。结果 小蘖碱的线性范围为 4 15～ 33 2 μg·ml- 1(r =0 9998) ,平均回收率为 98 7% ,RSD为 1 4 0 % (n =5)。结论 五批黄连中小蘖碱的含量测定结果表明两种测定方法具有相同的准确度。因此 ,酸性染料比色法也可用于黄连中小蘖碱的含量测定
Objective To find a simple and applicable method for the determination of Coptis chinensis. Methods The content of berberine in Coptis chinensis was determined by acid dye colorimetry and thin layer chromatography scanning. Results The linear range of berberine was 4 15 to 33 2 μg·ml - 1 (r = 9998). The average recovery was 98 7% and the RSD was 14% (n = 5). Conclusion The results of the determination of berberine in five batches of Coptis chinensis show that the two methods have the same accuracy. Therefore, acid dye colorimetry can also be used to determine the content of berberine in Coptis chinensis.