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目的:检测精子相关抗原5（sperm-associated antigen 5，SPAG5）在胰腺癌组织中的表达情况，初步探究SPAG5与胰腺癌的临床预后关系。探讨SPAG5作为胰腺癌潜在生物标志物的可能性。方法:用生物信息学的方法分析SPAG5在胰腺癌组织及癌旁正常组织中的mRNA水平，并分析其表达与胰腺癌患者的生存率间的关系。回顾性分析94例接受手术治疗的胰腺癌患者的临床病理资料。采用免疫组化法检测胰腺癌组织和癌旁正常组织中的SPAG5蛋白表达水平，分析其与胰腺癌患者临床病理特征的关系。结果:生物信息学分析结果显示，SPAG5的mRNA在胰腺癌组织中显著高表达，并与患者总生存率和无病生存率显著相关。免疫组化结果表明，SPAG5在胰腺癌组织中的表达明显高于癌旁正常组织。SPAG5在胰腺癌组织中的高表达与肿瘤分期、肿瘤尺寸及淋巴结的转移情况相关（均n P0.05）。n 结论:SPAG5的表达量与胰腺癌的进展显著相关，标靶SPAG5或可成为胰腺癌的一个新的治疗方法。SPAG5可能作为胰腺癌的一个新的潜在生物标志物。“,”Objective:To detect the expression of sperm-associated antigen 5 (SPAG5) in pancreatic cancer tissues, and to explore the relationship between SPAG5 and the clinical prognosis of pancreatic cancer. To explore the possibility of SPAG5 as a potential biomarker for pancreatic cancer.Methods:The bioinformatics method was used to analyze the mRNA level of SPAG5 in pancreatic cancer tissues and normal tissues adjacent to the cancer, and to analyze the relationship between its expression and the survival rate of pancreatic cancer patients. The clinicopathological data of 94 patients with pancreatic cancer who underwent surgical treatment were retrospectively analyzed. Immunohistochemical method was used to detect the expression level of SPAG5 protein in pancreatic cancer tissues and normal tissues adjacent to the cancer, and to analyze its relationship with clinicopathological characteristics of patients with pancreatic cancer.Results:The results of bioinformatics analysis showed that SPAG5 mRNA is significantly highly expressed in pancreatic cancer tissues and is significantly correlated with the overall survival rate and disease-free survival rate of patients. The results of immunohistochemistry showed that the expression of SPAG5 in pancreatic cancer tissues was significantly higher than that in normal tissues adjacent to cancer. The high expression of SPAG5 in pancreatic cancer tissue is related to tumor stage, tumor size and lymph node metastasis (all n P0.05).n Conclusions:The expression of SPAG5 is significantly related to the progression of pancreatic cancer. Targeting SPAG5 may become a new treatment method for pancreatic cancer. SPAG5 may serve as a new potential biomarker for pancreatic cancer.