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用耐盐小麦品种永 4 4 5 (耐盐 1级 )作父本 ,与中肥水丰产品种原冬 3号杂交 ,采用单倍体、单细胞系统 ,用其 F1花药在 0 .4 % Na Cl浓度下诱导 ,进行第一步筛选 ,再在同样 Na Cl浓度下分化 ,作第二步筛选。获得 H89- 6和 H89- 7两个花粉植株 ,在非盐渍地生长繁殖一代 ,消除可能存在的生理适应性或后生遗传型耐盐表型。H3开始按穗行进行连续 3个世代的耐盐性田间鉴定 ,并进行单株选择。选出 H89- 7- 2 - 1系连续 3年耐盐性达到 1级 ,农艺性状较好 ,在 0 .73%含盐土壤上仍能生长 ,在含盐0 .17%～ 0 .73%的盐渍地上产量明显高于莱州 95 3品种。幼苗盐诱导脯氨酸累积过程的测定表明 ,在1.2 % Na Cl胁迫下 ,前 6d幼苗脯氨酸仅增至 2倍左右 ,第 8d后明显增加 ,至第 14d增至 13倍以上。
Using salt-tolerant wheat variety Yong445 (salt-tolerant grade 1) as the male parent and cross fertilization with Zhongfeishuihui variety Yuanyuan3, using the haploid and single-cell system, F1 anthers were used in 0.4% Na Cl concentration induced, the first screening, and then differentiated in the same Na Cl concentration for the second screening. Two pollen plants, H89-6 and H89-7, were obtained, which grew and propagated in non-salted soil to eliminate the possible physiological adaptability or epigenetic salt-tolerant phenotype. H3 began three lines of salt tolerant field identification on a row-by-row basis and single plant selection. The results showed that the salt tolerance of H89- 7- 2 - 1 was up to Grade 1 for 3 consecutive years and its agronomic traits were good. It could still grow on 0.73% saline soil, with the salt content of 0.17% -0.73% The salinity aboveground yield was significantly higher than Laizhou 95 3 variety. The proline accumulation induced by salt-induced seedlings showed that the proline content in the first 6 days of seedlings increased only about 2-fold under 1.2% NaCl stress and increased significantly after 8 days, reaching 13-fold above the 14th day.