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以对黑腐病抗性不同的2个花椰菜(B rassica oleracea var.botry tis)品种为材料,研究了花椰菜苗期抵抗黑腐病的生理机制。结果表明:接种7 d后,黑腐病菌(X anthom onas camp estris pv.C amp estris)的侵入导致花椰菜幼苗干物质积累下降,抗病品种‘雪峰’的下降幅度明显低于感病品种‘2003X-106’,这与接种3 d后雪峰的净光合速率、气孔导度、蒸腾速率和叶绿素含量等下降较慢有关。病原菌侵染后,2个品种叶片的可溶性糖含量均有增加,但是抗病品种‘雪峰’的增幅较感病品种‘2003X-106’低;‘雪峰’叶片的可溶性蛋白质含量在接种后逐渐降低,而‘2003X-106’却逐渐增加;接种后,‘雪峰’叶片的IAA和M e-JA含量均上升,ABA则显著降低,而‘2003X-106’的IAA含量降低,ABA显著增加,M e-JA则在接种后的不同时期有增有减。
Two Cauliflower (Brassica oleracea var. Botry tis) varieties with different resistance to black rot were used to study the physiological mechanism of cauliflower resistance to black rot at seedling stage. The results showed that after 7 days of inoculation, the invasion of X anthom onas camp estris pv. C amp estris resulted in the decrease of dry matter accumulation of cauliflower seedlings, the decrease of ’Xuefeng’ of resistant varieties was significantly lower than that of susceptible varieties’ 2003X-106 ’, which is related to the slow decline of net photosynthetic rate, stomatal conductance, transpiration rate and chlorophyll content of Xuefeng 3 days after inoculation. After the pathogen invaded, the soluble sugar content increased in both cultivars, but the resistant cultivar ’Xuefeng’ had a lower growth rate than the susceptible cultivar ’2003X-106’. The content of soluble protein in ’Xuefeng’ While the content of IAA and M e-JA in ’Xuefeng’ leaves increased and ABA decreased significantly, but IAA content in ’2003X-106’ decreased and ABA was significant M e-JA increased or decreased at different stages after inoculation.