本文采用简便的HPLC条件和萃取方法，12min内同时测定微量血清（100ul）中的苯巴比妥、苯妥英和卡马西平的含量．以苯乙酮为内标，样品用三氯甲烷萃取，N260℃吹干．流动相为McOH／H2O（55／45，V／V），流速1．0ml／min，于220nm检测．在7个不同水平，PBT、PHT、CBZ的平均回收率为99．39％、98．35％和99．69％，天内和天间变异均低于5％，在分析范围内有良好的线性关系．应用本法测定了8名病人血清中的药物浓度． In this paper, simple HPLC conditions and extraction methods, simultaneous detection of trace amounts of phenobarbital, phenytoin and carbamazepine in 100ul in 12min. Acetophenone as the internal standard, the sample was extracted with chloroform, N260 ℃ dry. The mobile phase was McOH / H2O (55/45, V / V) and the flow rate was 1.0 ml / min at 220 nm. At seven different levels, the average recoveries of PBT, PHT and CBZ were 99.39%, 98.35% and 99.69%, respectively. The average intra-and inter-day variation was less than 5% relationship. This method was used to determine the serum concentrations of eight patients.