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目的为阐明益生菌抗氧化与结肠炎的关系,对植物乳杆菌ZDY2013与两歧双歧杆菌WBIN03缓解三硝基苯磺酸(trinitro-benzene-sulfonic acid,TNBS)诱导的小鼠结肠炎进行探究。方法通过对BALB/c小鼠肛门注射TNBS,构建小鼠结肠炎模型;分别采用植物乳杆菌ZDY2013与两歧双歧杆菌WBIN03的单菌悬液(10~9CFU/mL)及1:1混合菌悬液(10~9CFU/mL)进行8 d灌胃治疗。结果治疗组小鼠结肠组织炎性细胞浸润症状获得缓解,血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)(t_1=3.247,P_1<0.05;t_2=3.397,P_2<0.05)、过氧化氢酶(catalase,CAT)(t_1=5.289,P1<0.001;t_2=3.563,P_2<0.05)和总超氧化物歧化酶(total superoxide dismutase,T-SOD)(t_1=3.317,P_1<0.05;t_2=3.551,P_2<0.05)活性均有显著恢复。结论植物乳杆菌ZDY2013与两歧双歧杆菌WBIN03可通过增强机体抗氧化酶活性,起到缓解TNBS诱导的小鼠结肠炎的作用。
Objective To elucidate the relationship between probiotic antibiotic and colitis, we investigated the protective effects of Lactobacillus plantarum ZDY2013 and Bifidobacterium bifidus WBIN03 on mouse colitis induced by trinitro-benzene-sulfonic acid (TNBS) . Methods BALB / c mice were injected intranasally with TNBS to establish a model of colitis in mice. Single colonies (10-9 CFU / mL) of Lactobacillus plantarum ZDY2013 and Bifidobacterium bifidum WBIN03 and 1: 1 mixed bacteria Suspension (10 ~ 9CFU / mL) for 8 d gavage treatment. Results The inflammatory cell infiltration in the colon of mice in the treatment group was relieved. The levels of GSH-PX (t 1 = 3.247, P 1 <0.05; t 2 = 3.397, P 2 <0.05) The levels of catalase (CAT) (t_1 = 5.289, P1 <0.001; t_2 = 3.563, P_2 <0.05) and the total superoxide dismutase (T-SOD) ; t 2 = 3.551, P 2 <0.05) showed significant recovery. Conclusion Lactobacillus plantarum ZDY2013 and Bifidobacterium bifidum WBIN03 can relieve the colitis induced by TNBS in mice by increasing the body’s antioxidant enzyme activity.