为了研究大蒜的药理作用 ,从大蒜中分离得到了含硫的简单有机化合物阿藿烯 (z_Ajoene) .在体外以不同浓度的阿藿烯处理 3种不同的肿瘤细胞 ,应用MTT法分析阿藿烯对肿瘤细胞生长的影响 ,采用光学显微镜、流式细胞仪和琼脂糖凝胶电泳等技术分析肿瘤细胞形态、DNA含量和染色体DNA的断裂 ,使用West ern蛋白印迹法测定基因表达 .发现阿藿烯对HL_6 0 ,MGc_80 3和Molt_4等 3种肿瘤细胞均具有明显的致凋亡作用 ,其机制可能是通过抑制原癌基因bcl_2的表达而实现的 . In order to study the pharmacological effects of garlic, a simple organic compound containing sulfur, z_Ajoene, was isolated from garlic. Three different types of tumor cells were treated with different concentrations of adecene in vitro. Adecene was analyzed by MTT assay. The influence of tumor cell growth was analyzed by optical microscopy, flow cytometry and agarose gel electrophoresis on the morphology, DNA content, and chromosomal DNA breaks. Western blotting was used to determine gene expression. The three tumor cells of HL_6 0, MGc_80 3 and Molt_4 have obvious apoptosis-inducing effect. The mechanism may be achieved by inhibiting the expression of proto-oncogene bcl_2.