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目的观察连续传代的铜绿假单胞菌临床株对邻苯二甲醛和碘伏的抗性变化及其与qacE-sul1基因的关系。方法采用肉汤稀释法、悬液定量杀菌试验和PCR方法,检测了两种消毒剂对连续传代的铜绿假单胞菌临床株最小抑菌浓度、最小杀菌浓度变化和抗药基因携带率。结果临床分离的铜绿假单胞菌经连续传代后对邻苯二甲醛、碘伏的抗性均逐渐增强,这些菌株qacE-sul1基因检测结果均为阴性。结论长期使用亚致死剂量的消毒剂可能导致铜绿假单胞菌的抗性产生或增强,其抗性变化与qacE-sul1基因无关。
Objective To investigate the changes of resistance to o-phthalaldehyde and iodophor and their relationship with qacE-sul1 gene in continuous passage of Pseudomonas aeruginosa clinical strains. Methods The minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MIC) and the rate of drug-resistant gene carrier of two kinds of disinfectants on the continuous passage of Pseudomonas aeruginosa clinical strains were tested by broth dilution method, quantitative bactericidal test and PCR method. Results The clinical isolates of P. aeruginosa were continuously passaged after the resistance of o-phthalaldehyde and iodophor were gradually increased, and the results of qacE-sul1 gene of these strains were negative. CONCLUSIONS Long-term use of sublethal doses of disinfectants may result in the production or enhancement of resistance to P. aeruginosa, with changes in resistance not related to the qacE-sul1 gene.