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目的探讨儿茶素的主要成分L-表没食子基儿茶素没食子酸酯(L-EGCG)对脂蛋白(a)[Lp(a)]诱导的大鼠肾小球系膜细胞(GMCs)增生影响及可能机制。方法选定最佳剂量与最佳时间将GMCs分为对照组、Lp(a)组[Lp(a)(5.0μg/ml)]、L-EGCG组[Lp(a)(5.0μg/ml)+L-EGCG(200mg/L)处理]共三组分瓶培养。观察L-EGCG对GMCs增生率(MTT)、增生细胞核抗原(PCNA)阳性表达率及培养液上清的细胞间粘附因子-1(ICAM-1)浓度的影响,并与对照组相比较。结果在对照组、Lp(a)组及L-EGCG组,随着处理时间的延长,GMCs的MTT、PCNA、上清ICAM-1浓度呈增加趋势,经F检验,差异有统计学意义(P<0.01)。与对照组比较,应用Lp(a)5.0g/ml处理后,在不同的处理时间,Lp(a)组MTT计数、PCNA阳性率、ICAM-1均明显增高,经两两之间的q检验,差异有统计学意义(P<0.01)。与Lp(a)组比较,应用L-EGCG处理Lp(a)诱导的大鼠GMCs,在不同的处理时间,L-EGCG组MTT计数、PCNA阳性率、ICAM-1均明显降低,经两两之间的q检验,差异有统计学意义(P<0.01)。在Lp(a)组、L-EGCG组,培养上清ICAM-1浓度与PCNA阳性率及与反映系膜细胞增生指标的MTT呈正相关。结论Lp(a)能明显促进肾脏GMCs的增生,L-EGCG可明显抑制Lp(a)诱导的大鼠GMCs增生,此可能与影响ICAM-1表达有关。
Objective To investigate the effects of L-epigallocatechin gallate (L-EGCG), the main component of catechin, on the proliferation of glomerular mesangial cells (GMCs) induced by lipoprotein (a) [Lp Impact and possible mechanisms. Methods GMCs were divided into control group, Lp (a) (5.0μg / ml)] and L-EGCG group [Lp (a) + L-EGCG (200 mg / L)]. The effect of L-EGCG on the expression of GMCs proliferation rate (MTT), PCNA positive rate and ICAM-1 concentration in culture supernatants was observed and compared with the control group. Results In the control group, Lp (a) group and L-EGCG group, the concentrations of MTT, PCNA and supernatant ICAM-1 in GMCs tended to increase with the prolongation of treatment time, and the difference was statistically significant <0.01). Compared with the control group, MTT counts, PCNA positive rate and ICAM-1 in Lp (a) group were significantly increased at different treatment time after treatment with 5.0g / ml Lp (a) , The difference was statistically significant (P <0.01). Compared with Lp (a) group, L-EGCG treatment Lp (a) -induced GMCs in rats, MTT count, PCNA positive rate, ICAM-1 were significantly decreased in L-EGCG group at different treatment time, Between the q test, the difference was statistically significant (P <0.01). In Lp (a) group, the concentration of ICAM-1 in L-EGCG group and culture supernatant was positively correlated with the positive rate of PCNA and the MTT with mesangial cell proliferation index. Conclusions Lp (a) can significantly promote the proliferation of GMCs in kidneys. L-EGCG can significantly inhibit Lp (a) -induced proliferation of GMCs in rats, which may be related to the effect of ICAM-1 expression.