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目的:观察长链非编码RNA肝细胞核因子1α反义链1(lnc HNF1A-AS1)对食管癌细胞增殖、迁移和凋亡的影响并探讨其作用机制。方法:选取2017年8月至2019年11月于河南省人民医院行根治性切除术的食管鳞癌患者85例作为研究对象，采用反转录-聚合酶链反应(RT-PCR)检测食管癌和癌旁组织lnc HNF1A-AS1的mRNA表达水平；构建短发卡RNA(shRNA)-Control和shRNA-HNF1A-AS1慢病毒稳定细胞系；采用生物信息学和双荧光素酶报告基因实验分析lnc HNF1A-AS1和miR-1-3p的靶向关系；采用细胞计数试剂盒(CCK-8)法检测shRNA-Control和shRNA-HNF1A-AS1细胞的增殖水平；采用Transwell检测shRNA-Control和shRNA-HNF1A-AS1细胞的迁移能力；采用原位缺口末端标记法(TUNEL)染色检测shRNA-Control和shRNA-HNF1A-AS1细胞的凋亡水平；采用蛋白质印迹法(Western blot)检测细胞周期蛋白D1(Cyclin D1)、程序性死亡因子10(PDCD10)的表达；组间比较采用n t检验及n χ2检验。n 结果:食管癌组织(1.77±0.10)lnc HNF1A-AS1相对表达水平高于癌旁组织(0.39±0.02)(n t＝7.294，n P<0.05)，差异有统计学意义。生物信息学分析显示lnc HNF1A-AS1是miR-1的潜在作用靶点。shRNA-HNF1A-AS1细胞吸光度(n A)值(0.51±0.03)低于shRNA-Control细胞n A值(1.65±0.11)，差异有统计学意义(n t＝4.904，n P<0.05)。shRNA-HNF1A-AS1细胞[(62.08±5.96)个]的迁移数目低于shRNA-Control细胞[(150.44±10.21)个]，差异有统计学意义(n t＝3.846，n P<0.05)。shRNA-HNF1A-AS1细胞[(83.85±6.69)个]的凋亡数量高于shRNA-Control细胞[(25.92±4.08)个]，差异有统计学意义(n t＝4.759，n P<0.05)。shRNA-HNF1A-AS1细胞中Cyclin D1和PDCD10的蛋白相对表达水平(0.55±0.05、0.59±0.04)低于shRNA-Control细胞(1.68±0.09、1.80±0.11)，差异有统计学意义(n t＝4.363、5.063，n P<0.05)。miR-1-3p组细胞Cyclin D1和PDCD10的蛋白相对表达水平(0.38±0.04、0.44±0.05)低于shRNA-Control细胞(1.76±0.10、1.72±0.12)，差异有统计学意义(n t＝5.176、4.729，n P<0.05)。n 结论:lnc HNF1A-AS1可能通过靶向下调miR-1-3p的表达，促进食管癌细胞的增殖和迁移，抑制食管癌细胞的凋亡。“,”Objective:To observe the effect of long non-coding RNA hepatocyte nuclear factor 1α-antisence 1 (lnc HNF1A-AS1) on proliferation, migration and apoptosis of esophageal cancer cells.Methods:A Total of 85 patients with esophageal squamous cell carcinoma who underwent radical resection in Henan Provincial People′s Hospital from August 2017 to November 2019 were selected as the research objects. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of lnc HNF1A-AS1 in esophageal cancer and adjacent tissues. Short hairpin RNA (shRNA)-control and shRNA-HNF1A-AS1 lentivirus stable cell lines were constructed. Bioinformatics and dual luciferase reporter gene experiment were used to analyze the targeting relationship between lnc HNF1A-AS1 and miR-1-3p. The proliferation activity of shRNA-control and shRNA-HNF1A-AS1 cells was detected by cell counting kit-8 (CCK-8) assay. Transwell assay was used to detect the cell migration. TUNEL staining was used to detect the apoptosis. Western blotting was used to detect the expression of Cyclin D1 and PDCD10. SPSS 17.0 statistical software was used to analyze the data with normal distribution, and the data were expressed as mean±standard deviation.Results:The relative expression level of LNC HNF1A-AS1 in esophageal carcinoma (1.77±0.10) was higher than that in adjacent tissues (0.39±0.02, n t=7.294, n P<0.05). Bioinformatics analysis showed that LNC HNF1A-AS1 was a potential target of miR-1-3p. The absorbance value of shRNA-HNF1A-AS1 cells (0.51±0.03) was significantly lower than that of shRNA control cells (1.65±0.11,n t=4.904, n P<0.05). The migration number of shRNA-HNF1A-AS1 cells [(62.08±5.96) cells] was significantly less than that of shRNA control cells [(150.44±10.21) cells,n t=3.846, n P<0.05]. The number of apoptotic shRNA-HNF1A-AS1 cells [(83.85±6.69) cells] was significantly greater than that in shRNA control cells [(25.92±4.08) cells,n t=4.759, n P<0.05]. The relative protein expression levels of Cyclin D1 and PDCD10 in shRNA-HNF1A-AS1 cells (0.55±0.05, 0.59±0.04 cells) were significantly lower than those in shRNA control cells (1.68±0.09, 1.80±0.11,n t=4.363, 5.063, n P<0.05). The relative protein expression levels of Cyclin D1 and PDCD10 in miR-1-3p group (0.38±0.04, 0.44±0.05) were significantly lower than those in shRNA control cells (1.76±0.10, 1.72±0.12,n t=5.176, 4.729, n P<0.05).n Conclusion:Lnc HNF1A-AS1 may promote the proliferation and migration of esophageal cancer cells and inhibit the apoptosis of esophageal cancer cells by targeting down-regulation of miR-1-3p expression.