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Objective To investigate the effects of pharmacologic ascorbate(vitamin C)against Influenza A/CA/7/09(H1N12009).Methods BALB/c mice inoculated intranasally with influenza virus were treated with ascorbate(3 mg/g)twice daily by intraperitoneal(i.p.)injection for up to 14 d.Control groups received an equivalent volume of normal saline.Body weight was measured daily.To quantify the level of viral replication in the respiratory tract,the mice were euthanized and lungs removed and prepared as whole lung homegenates.Viral titers were determined by TCID50assay in MDCK cells.Cytokine titers were determined by ELISA following the
Objective To investigate the effects of pharmacologic ascorbate (vitamin C) against Influenza A / CA / 7/09 (H1N12009) .Methods BALB / c mice inoculated intranasally with influenza virus were treated with ascorbate (3 mg / g) twice daily by intraperitoneal ip) injection for up to 14 d. Control groups received an equivalent volume of normal saline. Body weight was measured daily. To quantify the level of viral replication in the respiratory tract, the mice were euthanized and lungs removed and prepared as whole lung home genates .Viral titers were determined by TCID50assay in MDCK cells. Cytokine titers were determined by ELISA following the