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作者将合诺氏疟原虫的环状体、滋养体和裂殖体的红细胞分别混合于等量的二甲亚砜中(200毫升/升DMSO),将此血样直接浸入液氮内(一步法)或者先置于-25～-32C中5、10、30或60分钟后,再置于-196C的液氮内(二步法),以观察二种方法对不同时期的红细胞内期原虫的保存作用。结果发现,当用20%感染红细胞,其中96%为滋养体和裂殖体的血样作观察时,一步法对滋养体和裂殖体都有严重的损害,而二步法的血样,即先
The authors mixed erythrocytes of the loop, trophozoites and schizonts of Plasmodium knowlesi with equal amounts of dimethylsulfoxide (200 ml / l of DMSO) and immersed them directly in liquid nitrogen (one-step method ) Or placed in -25 ~ -32C in 5,10,30 or 60 minutes, and then placed in -196C liquid nitrogen (two-step method) to observe the two methods of different stages of erythrocytic protozoan Save effect. The results showed that when 20% of erythrocytes were infected, 96% of which were trophozoites and schizonts, the one-step method had severe damage to trophozoites and schizonts, whereas the two-step method of sampling