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目的建立液质联用法测定Beagle犬血浆中去甲文拉法辛的质量浓度,并应用该方法研究琥珀酸去甲文拉法辛缓释片在Beagle犬体内的药代动力学行为。方法采用液液萃取法处理血浆样品,色谱柱选择Venusil ASB C_(18)柱,以甲醇-浓度5 mmol·L~(-1)醋酸铵缓冲溶液(体积比35∶65)为流动相,采用多反应离子监测(multiple reaction monitoring,MRM)扫描方式进行检测。结果去甲文拉法辛的质量浓度在0.50~100μg·L~(-1)内与峰面积线性关系良好,相关系数r为0.999 0,日内和日间精密度RSD≤6.2%,去甲文拉法辛的提取回收率在79.6%~83.3%内,基质效应在96.5%~98.8%内。结论本方法适用于琥珀酸去甲文拉法辛缓释片在Beagle犬体内药代动力学行为的研究。
Objective To establish a method for the determination of desvenlafaxine in Beagle dogs by liquid chromatography-mass spectrometry (LC-MS / MS) and to study the pharmacokinetics of desvenlafaxine succinate sustained-release tablets in Beagle dogs. Methods The plasma samples were extracted by liquid - liquid extraction. Venusil ASB C 18 column was used as the mobile phase. The mobile phase consisted of methanol - 5 mmol·L -1 ammonium acetate buffer solution (volume ratio 35:65) Multiple reaction monitoring (MRM) scanning was performed. Results The concentration of desvenlafaxine had a good linear relationship with the peak area in the range of 0.50 ~ 100 μg · L -1, the correlation coefficient r was 0.999 0, the intra-and / or intra-day precision RSD was ≤6.2% The extraction recovery of Lafaxin was between 79.6% and 83.3%, and the matrix effect was within 96.5% ~ 98.8%. Conclusion This method is suitable for the pharmacokinetics of desvenlafaxine succinate sustained-release tablets in Beagle dogs.