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目的探讨人脑胶质瘤中12-脂氧合酶(12-LOX)的表达及与病理、临床特征等的关系。方法选取2011年2月至2015年10月首次手术治疗的30例脑胶质瘤患者(脑胶质瘤组)为研究对象,以行颅内减压术的患者20例为对照组;对胶质瘤组手术切除的瘤体组织和对照组颅内减压术获得的正常脑组织,采用免疫组化方法检测和对比12-LOX的表达,分析其表达和胶质瘤患者病理及临床特征的关系。结果 (1)30例胶质瘤患者标本中,23例(76.7%)呈现12-LOX的强阳性表达,7例为弱阳性;而20例对照组患者正常脑组织均为弱阳性表达;两者强阳性表达率差异有统计学意义(P<0.01)。(2)脑胶质瘤患者的性别、年龄、病灶直径、反映肿瘤患者功能状态的KPS评分均与12-LOX的表达无显著关联(P均>0.05);12-LOX的表达与胶质瘤病理级别相关,随着恶性程度升高,12-LOX的强阳性率表现出递增趋势(P<0.01)。(3)弱阳性表达12-LOX的脑胶质瘤患者其生存率显著高于强阳性表达患者(P<0.01)。结论脑胶质瘤组织存在异常12-LOX高表达,脑胶质瘤患者12-LOX表达和病理分级关系密切,与患者预后相关。
Objective To investigate the expression of 12-lipoxygenase (12-LOX) in human glioma and its relationship with pathology, clinical features and so on. Methods Thirty patients with glioma (glioma group) who underwent the first operation from February 2011 to October 2015 were enrolled in this study. Twenty patients undergoing intracranial decompression were selected as the control group. The tumor tissue of the tumor group and the normal brain tissue obtained from the control group undergoing intracranial decompression were used to detect and compare the expression of 12-LOX by immunohistochemistry. The expression of 12-LOX and the pathological and clinical features of gliomas relationship. Results (1) Of the 30 glioma patients, 23 cases (76.7%) showed strong positive expression of 12-LOX, 7 cases were weakly positive, while 20 cases of normal control group were weakly positive brain tissue expression; two Strong positive expression rate of the difference was statistically significant (P <0.01). (2) There was no significant correlation between the expression of 12-LOX and the gender, age, lesion diameter and KPS score of glioma patients (all P> 0.05). The expression of 12-LOX was correlated with glioma The pathological grade was correlated. With the increase of malignancy, the strong positive rate of 12-LOX showed an increasing trend (P <0.01). (3) The survival rate of glioma patients with weak positive expression of 12-LOX was significantly higher than that of patients with strong positive expression (P <0.01). Conclusion The abnormal expression of 12-LOX exists in glioma tissue. The expression of 12-LOX in glioma is closely related to the pathological grade, which is correlated with the prognosis of patients.