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目的探讨体外5-氮杂-2′-脱氧胞苷(5-AzadC)、曲古抑菌素A(TSA)联合5-氟尿嘧啶(5-FU)和紫杉醇(PTX)对人胃癌SGC-7901细胞生长及6-氧-甲基鸟嘌呤-DNA甲基转移酶(MGMT)基因表达的影响。方法将培养的SGC-7901细胞分为八组:对照组、5-FU+PTX组、5-AzadC组、TSA组、5-AzadC+TSA组、5-AzadC+5-FU+PTX组、TSA+5-FU+PTX组和5-AzadC+TSA+5-FU+PTX组。用MTS法测定各组药物对SGC-7901细胞生长的影响,RT-PCR和Western blot方法检测SGC-7901细胞MGMT mRNA及其蛋白的表达。结果 5-AzadC、TSA、5-FU+PTX均能抑制细胞生长,且呈时间依赖性,多药联合组较单药组抑制率增强(P<0.05);加药72h后多药联合组较单药组MGMT mRNA和蛋白表达增强(P<0.05)。结论 5-AzadC、TSA、5-FU+PTX及药物联合干预通过增强MGMT基因再表达而抑制人胃癌SGC-7901细胞的生长。
Objective To investigate the effects of 5-AzadC, TSA combined with 5-fluorouracil and paclitaxel on human gastric cancer cell line SGC-7901 Growth and 6-O-Methylguanine-DNA Methyltransferase (MGMT) Gene Expression. Methods SGC-7901 cells were divided into eight groups: control group, 5-FU + PTX group, 5-AzadC group, TSA group, 5-AzadC + TSA group, 5-AzadC + 5- + 5-FU + PTX group and 5-AzadC + TSA + 5-FU + PTX group. The effects of different drugs on the growth of SGC-7901 cells were determined by MTS method. The expressions of MGMT mRNA and protein in SGC-7901 cells were detected by RT-PCR and Western blot. Results The 5-AzadC, TSA, 5-FU + PTX could inhibit the cell growth in a time-dependent manner. The multi-drug combination group was more effective than the single drug group (P <0.05) The single drug group MGMT mRNA and protein expression increased (P <0.05). Conclusion Combined treatment of 5-AzadC, TSA, 5-FU + PTX and drugs can inhibit the growth of human gastric cancer cell line SGC-7901 by enhancing the re-expression of MGMT gene.