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目的检测珍珠番石榴、鸡心柿和番荔枝中多酚物质的种类和含量,了解对常见致病菌的抗菌活性。方法用高效液相色谱法测定水果提取液中多酚物质的成份,用微量稀释法测定对蜡样芽胞杆菌、金黄色葡萄球菌、白色念珠菌、表皮葡萄球菌、鼠伤寒沙门菌、福氏志贺菌、大肠埃希菌、铜绿假单胞菌等8种常见致病菌的最小抑菌浓度(minimal inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC)。结果三种热带水果提取液中均检出儿茶素、没食子酸、槲皮素、木犀草素等多酚物质,珍珠番石榴含儿茶素为391.93 mg/kg,鸡心柿和番荔枝含没食子酸分别为377.11 mg/kg和256.52 mg/kg。珍珠番石榴提取液对表皮葡萄球菌的抗菌活性最强,MIC为0.0781 g/m L;鸡心柿提取液对金黄色葡萄球菌的MIC为0.312 5 g/m L;提取液对受试菌具有明显的杀菌或抑菌作用,抗菌活性强弱为:G+>G-,球菌>杆菌>真菌,条件致病菌>致病菌。结论珍珠番石榴、鸡心柿和番荔枝所含的多酚物质具有较高的抗菌活性,其中含量最高的是儿茶素和没食子酸。
Objective To detect the types and contents of polyphenols in pearl guava, chickpea and annona and understand the antibacterial activity against common pathogenic bacteria. Methods The contents of polyphenols in fruit extract were determined by high performance liquid chromatography (HPLC). The contents of polyphenols in fruit extract were determined by micro-dilution method. Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 8 kinds of common pathogens such as Escherichia coli, Escherichia coli and Pseudomonas aeruginosa. Results The catechins, gallic acid, quercetin, luteolin and other polyphenols were detected in three kinds of tropical fruit extracts. The content of catechins in pearl guava was 391.93 mg / kg, The acids were 377.11 mg / kg and 256.52 mg / kg, respectively. The antibacterial activity of pearl guava extract against Staphylococcus epidermidis was the strongest with MIC of 0.0781 g / m L; the MIC of sycamore extract against Staphylococcus aureus was 0.312 5 g / m L; Bactericidal or bacteriostatic effect, the strength of antibacterial activity: G +> G-, cocci> bacilli> fungi, opportunistic pathogens> pathogenic bacteria. Conclusion The polyphenols contained in pearl guava, chixia and annona have higher antibacterial activities, among which catechin and gallic acid are the most abundant.