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用HPLC方法对湘西杜鹃花植物根、茎中的 15种氨基酸的含量进行了测定 .测定的结果是 ,根中氨基酸的质量分数为 12 .74‰ (干样 ) ,其中必需氨基酸为 5 .30‰ ,占 41.6 0 % ;茎中氨基酸的质量分数为 8.41‰ (干样 ) ,其中必需氨基酸为3.2 5‰ ,占 41.85 % .该方法检测效果好 ,平均回收率达 93.6 % ,氨基酸保留时间变异系数平均为 1.0± 0 .4% ,其峰面积变异系数为 4.2± 0 .8% ,在 10pmol～ 2nmol范围内 ,线性关系系数平均为 0 .987± 0 .0 0 9.
The content of 15 kinds of amino acids in root and stem of Rhododendron officinalis plantation in Xiangxi was determined by HPLC method.The results showed that the mass fraction of amino acid in root was 12.74 ‰ (dry sample), and the essential amino acid was 5.30 ‰, accounting for 41.6%; the mass fraction of amino acids in stems was 8.41 ‰ (dry sample), of which the essential amino acids were 3.2 5 ‰, accounting for 41.85% .The detection method was good, the average recovery rate was 93.6%, amino acid retention time variation The average coefficient of variation was 1.0 ± 0.4% and the coefficient of variation of peak area was 4.2 ± 0.8%. The average linearity coefficient was 0 .987 ± 0 .0 9 in the range of 10 pmol ~ 2 nmol.