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目的分析华东地区宫颈非角化型鳞状细胞癌组织中人乳头状瘤病毒(HPV)感染基因型的分布及其分型数据库建立的意义。方法采用基因扩增结合基因芯片技术对506例宫颈非角化型鳞状细胞癌组织标本进行23种HPV分型检测,并对患者的相关资料进行分析。结果 506例宫颈非角化型鳞状细胞癌检出HPV感染者456例,总的HPV感染率为90.1%,其中一型感染率为75.9%(384/506),多型感染率为14.3%(72/506)。结论宫颈非角化型鳞状细胞癌组织中,HPV以一型感染为主,多型感染为辅,出现频率最多的型别依次为16、18、33、58、52、31、59、45型,是致宫颈非角化型鳞状细胞癌的主流型别,16型尤其突出,占高危型出现频率的65%(344/529)。HPV基因芯片检测技术可用于宫颈鳞状细胞癌组织的分型检测,对我国女性宫颈癌的防治、HPV疫苗的研发以及宫颈癌组织HPV感染基因型数据库的建立都具有十分重要的意义。
Objective To analyze the distribution of human papillomavirus (HPV) genotypes in cervical non-keratinizing squamous cell carcinoma in East China and the significance of their genotyping database. Methods Twenty-three genotypes of cervical non-keratinizing squamous cell carcinoma were detected by genotyping and microarray in 23 types of HPV genotypes, and the related data of the patients were analyzed. Results A total of 456 cases of HPV were detected in 506 cases of cervical non-keratinizing squamous cell carcinoma. The total HPV infection rate was 90.1%, of which 75.9% (384/506) were type 1 infection and 14.3% (72/506). Conclusions In cervical non-keratinizing squamous cell carcinoma, HPV is predominantly type I and supplemented by polytype infection, with the most frequent types being HPV type 16,18,33,58,52,31,59,45 Type, is the leading type of cervical non-keratinizing squamous cell carcinoma, 16 type is particularly prominent, accounting for 65% of the frequency of high-risk (344/529). HPV gene chip detection technology can be used for cervical squamous cell carcinoma of the genotyping test for our prevention and treatment of cervical cancer in women, HPV vaccine research and development of cervical cancer tissue HPV genotype database has a very important significance.