用Southern Blot方法,我们研究了13份慢性HBV感染患者(8份慢迁旰,5份慢活肝)和2份对照精液标本中HBV DNA存在状况。提取物一半用限制酶HindⅢ消化,各行琼脂电泳,转移到纤维膜上,然后用~(32)P—HBV—DNA探针分子杂交。在4份精子內检出HBV DNA,3份为游离形式,1份为整合形式。结果表明HBV DNA可以存在精于内,游离状态HBV DNA提示有传染性、整合序列HBV DNA的存在提示有通过生殖细胞遗传的可能。 Using the Southern Blot method, we investigated the presence of HBV DNA in 13 chronic HBV-infected patients (8 slow-moving, 5 slow-moving) and 2 control semen samples. Half of the extract was digested with restriction enzyme HindIII and electrophoresed on each agar to transfer to the fiber membrane and then hybridized with ~ (32) P-HBV-DNA probe. HBV DNA was detected in 4 sperm, 3 as free form and 1 as an integrated form. The results show that HBV DNA can exist in the free and internal state of HBV DNA suggests contagious, the presence of integrated sequence HBV DNA suggests the possibility of germline genetics.