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采用液体培养法,以对水分敏感程度不同的两个青稞品种(旱地紫青稞和大麻青稞)为材料,研究PEG模拟干旱条件下青稞幼苗叶片诱导蛋白表达的差异。结果表明,干旱胁迫96h,经SDS-PAGE分析发现,在50k Da左右处有干旱诱导蛋白出现。经过质谱分析检测出这些蛋白分别参与植物的光合系统、糖代谢途径、抗氧化反应、蛋白合成、胁迫应激响应等生理过程。分析发现,对水分胁迫不敏感的旱地紫青稞在相同处理条件下可以启动更多蛋白的转录表达,以帮助植物应对环境变化。
A liquid culture method was used to study the differences of induced protein expression in leaves of barley seedlings under simulated drought stress in the condition of two varieties of barley (Azolla and Azadirachtoral) which are sensitive to water stress. The results showed that drought-induced 96h, by SDS-PAGE analysis found that about 50kDa drought induced protein appear. After mass spectrometry analysis, these proteins were involved in the physiological processes of photosynthesis, glycometabolism, antioxidation, protein synthesis and stress response in plants. The results showed that under the same treatment conditions, the transpiration expression of more proteins could be activated in dryland purplegrass, which is insensitive to water stress, to help plants to cope with environmental changes.