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目的:建立人腺样囊性癌耐药细胞系,为阐明腺样囊性癌的多药耐药机制及逆转耐药提供模型及研究依据。方法:以长春新碱(VCR)为诱导剂,通过浓度递增间断刺激法对人腺样囊性癌细胞系(ACC)进行体外诱导耐药,建立耐VCR的腺样囊性癌细胞系ACC/VCR。细胞计数法绘制细胞生长曲线,噻唑蓝(MTT)比色法检测细胞对化疗药物敏感性。结果:ACC经体外诱导后,ACC/VCR细胞对长春新碱(VCR)、阿霉素(ADM)和平阳霉素(PYM)的耐药性明显增强,具有交叉耐药,对环磷酰胺(CTX)、氟尿嘧啶(5-FU)和顺铂(DDP)耐药性无明显变化。耐药前后ACC细胞的生长曲线、群体倍增时间和光镜下的形态无明显改变。结论:VCR可诱导腺样囊性癌细胞产生多药耐药。
OBJECTIVE: To establish a human adenoid cystic carcinoma cell line that is resistant to multidrug resistance and to reverse drug resistance in adenoid cystic carcinoma. Methods: Vincristine (VCR) was used as an inducer to induce human adenoid cystic carcinoma cell line (ACC) in vitro by increasing concentration and intermittent stimulation. Adeno-resistant adenoid cystic carcinoma cell line ACC / VCR. The cell growth curve was drawn by cell counting method. The chemosensitivity of cells to chemotherapeutic drugs was detected by MTT assay. Results: After induced by ACC, the drug resistance of ACC / VCR cells to vincristine (VCR), doxorubicin (ADM) and bleomycin (PYM) was significantly enhanced and cross-resistant. CTX), fluorouracil (5-FU) and cisplatin (DDP) resistance did not change significantly. The growth curve of ACC cells before and after drug resistance, population doubling time and morphology under light microscope had no significant change. Conclusion: VCR can induce adenoid cystic carcinoma cells to produce multidrug resistance.