目的 :建立转移因子 (TF)等免疫增强制剂体外生物活性简便、快速的检测方法。方法 :利用溴化四唑蓝法测定TF等促进小鼠脾淋巴细胞的增殖活性。结果 :TF、胸腺因子、胎盘因子三者均能明显地促进淋巴细胞增殖 ,平均增殖率为 6 3 .5 1%、77.6 5 %、86 .6 1%。结论 :此法稳定可靠 ,易于掌握、普及。 OBJECTIVE: To establish a simple and rapid method for the in vitro biological activity of immune enhancement preparations such as transfer factor (TF). Methods: The proliferation activity of mouse splenic lymphocytes was assayed by TF tetrazolium bromide method. Results: TF, thymus factor and placental factor all significantly promoted the proliferation of lymphocytes. The average proliferation rates were 63.51%, 77.65% and 86.61%. Conclusion: This method is stable and reliable, easy to grasp and popularize.