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目的:初步研究ⅢA型慢性前列腺炎患者与健康男性前列腺按摩液中差异蛋白质的表达。方法:对ⅢA型慢性前列腺炎患者前列腺按摩液标本35例和正常成年无前列腺炎的男性前列腺按摩液标本18例,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF/MS)检测各组标本中差异表达的蛋白质,所得数据运用相关软件对不同蛋白质峰的质荷比(M/Z)进行统计分析,并运用蛋白质数据库对相关蛋白质进行检索分析。结果:ⅢA型慢性前列腺炎组和正常男性对照组比较,表达差异超过2倍的有5种蛋白质,其中M/Z在M r为3 372、3 487、4 257、5 325的蛋白质高表达(P<0.01),M/Z在M r为1 0631的蛋白质低表达(P<0.01)。这5种蛋白质可能分别为Brevinin-2Eg、巨大内皮素-1、α-防御素15、β-防御素134、前列腺甾体蛋白C2。结论:ⅢA型慢性前列腺炎患者与健康男性前列腺按摩液中蛋白质表达存在差异,这些蛋白质可能与ⅢA型慢性前列腺炎的发生、发展有重要相关性。
OBJECTIVE: To study the expression of differential proteins in patients with type ⅢA chronic prostatitis and healthy male prostate massage. Methods: A total of 18 specimens of prostatic massage specimens from patients with type Ⅲ A chronic prostatitis and 18 specimens from normal male prostates without prostatitis were collected. MALDI-TOF / MS was used to detect the specimens In the data obtained, the software was used to statistically analyze the mass-to-charge ratio (M / Z) of different protein peaks, and the related proteins were searched and analyzed by using the protein database. Results: There were five proteins with more than 2-fold difference in the expression of M / Z in the chronic prostatitis group ⅢA compared with the normal male control group. Among them, M / Z protein was highly expressed in the M r of 3 372, 3 487, 4 257, 5 325 P <0.01). M / Z protein was low expressed at M r of 1 0631 (P <0.01). The five proteins may be Brevinin-2Eg, giant endothelin-1, alpha-defensin 15, beta-defensin 134, and prostate steroid protein C2. Conclusion: There is a difference in protein expression between type ⅢA chronic prostatitis and healthy male prostate massage. These proteins may be correlated with the occurrence and development of type ⅢA chronic prostatitis.