论文部分内容阅读
目的 探讨同种异体骨髓间充质干细胞 (MSC)在大鼠心脏定居存活情况、同种异体移植是否可行。正常心脏微环境与梗死心脏微环境对骨髓间充质干细胞 (MSC)迁移、分化的影响。方法 雌性Wistar大鼠 35只 ,①正常心脏MSC移植组 (1 0只 ) ;②急性心肌梗死对照组 (AMI,1 0只 ) ;③心肌梗死MSC移植组 (1 0只 ) ;④单个核细胞治疗组 (5只 )。结扎左冠状动脉前降支造成心肌梗死模型。分离纯化来自雄性Wistar大鼠的骨髓MSC ,于冠脉结扎后 1～ 3h植入到雌性正常大鼠和心肌梗死大鼠心脏组织 ,模拟同种异体细胞移植治疗。于细胞移植后 1 0周取心脏检测各种指标。结果(1 )经纯化的大鼠MSC在同种异体大鼠心脏可定居、生存 ,而未经纯化的单个核细胞移植在同种异体大鼠心脏未见存活 ;(2 )在正常心脏微环境中 ,带蓝色荧光的供体MSC细胞核呈小岛屿状分布 ,与宿主心肌纤维排列无关 ;而在心肌梗死模型大鼠心脏微环境中 ,蓝色细胞核分布广泛 ,呈椭圆形 ,似心肌细胞核 ,其排列方向与宿主心肌纤维排列方向一致 ,免疫组化检测胞浆心肌特异蛋白染色阳性。结论 纯化的MSC具有免疫耐受特性 ,无需加用免疫抑制剂 ,可进行同种异体移植 ;同种异体MSC在正常心脏微环境中不发生迁移、分化 ;而在急性梗死大鼠心脏中具有向缺血梗死区迁移并
Objective To investigate the survival and survival of allogeneic bone marrow mesenchymal stem cells (MSCs) in rat heart and whether allogeneic transplantation is feasible. Effects of normal cardiac microenvironment and infarcted cardiac microenvironment on the migration and differentiation of bone marrow mesenchymal stem cells (MSC). Methods Thirty-five female Wistar rats were randomly divided into three groups: ① normal cardiac MSC group (10); ② acute myocardial infarction control group (AMI, 10); ③ myocardial infarction MSC transplantation group (10); ④ mononuclear cells Treatment group (5). Ligation of left anterior descending coronary artery caused by myocardial infarction model. Isolation and purification of bone marrow MSC from male Wistar rats were implanted into the heart tissues of normal female rats and myocardial infarction rats 1 ~ 3h after coronary artery ligation, and the allogeneic cells transplantation was simulated. At 10 weeks after cell transplantation, the heart was taken to measure various indexes. Results (1) The purified rat MSCs could colonize and survive in allogeneic rat heart, but no purified mononuclear cells were transplanted in allogeneic rat heart. (2) In normal cardiac microenvironment , The nuclei of donor cells with blue fluorescence showed a small island distribution and had no relationship with the host myocardial fiber arrangement. In the myocardial microenvironment of myocardial infarction model, the blue nuclei were widely distributed and oval, The arrangement direction of the fibers was consistent with that of the host myocardium, and the immunohistochemical staining of cytoplasmic myocardial specific protein was positive. Conclusion The purified MSC possesses the characteristics of immune tolerance and can be allotransplantated without adding immunosuppressive agents. Allogeneic MSC do not migrate and differentiate in the normal cardiac microenvironment, while in the acute infarcted rat heart, Ischemic infarction area migration and