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探讨肺血管平滑肌细胞（ＰＶＳＭＣ）迁移在缺氧性肺血管结构重组中的作用以及缺氧本身对ＰＶＳＭＣ增殖和迁移的影响。方法利用细胞趋化研究方法和３Ｈ－胸腺嘧啶掺入法研究了血小板衍生生长因子（ＰＤＧＦ）对培养的新生小牛肺动脉平滑肌细胞（ＰＡＳＭＣ）趋化反应和ＤＮＡ合成的作用，以及缺氧和心钠素（ＡＮＰ）对ＰＤＧＦ这种作用的影响。结果表明缺氧可促进ＰＤＧＦ诱导的ＰＡＳＭＣ的趋化反应和ＤＮＡ合成，ＡＮＰ可抑制ＰＡＳＭＣ对ＰＤＧＦ的趋化作用，并抑制ＰＡＳＭＣ的ＤＮＡ合成，鸟苷酸环化酶抑制剂美蓝（ＭＢ）可抑制ＡＮＰ的这种抑制作用。结论研究提示ＰＤＧＦ、ＡＮＰ和缺氧本身对ＰＡＳＭＣ的增殖和迁移有重要作用，这可能对缺氧性肺血管结构重组具有重要意义
To investigate the role of pulmonary vascular smooth muscle cells (PVSMC) migration in hypoxic pulmonary vascular remodeling and the effect of hypoxia itself on the proliferation and migration of PVSMC. Methods The effects of platelet-derived growth factor (PDGF) on chemotactic response and DNA synthesis of cultured neonatal calf pulmonary artery smooth muscle cells (PASMCs) and DNA synthesis were studied by chemotaxis assay and 3H-thymidine incorporation. The Effect of Sodium Nitroprusside (ANP) on PDGF This Effect. The results showed that hypoxia promoted the chemotactic reaction and DNA synthesis of PASMC induced by PDGF. ANP inhibited the chemotactic effect of PASMC on PDGF and inhibited the DNA synthesis of PASMC. The guanylate cyclase inhibitor methylene blue (MB) Inhibit this inhibitory effect of ANP. Conclusions The results suggest that PDGF, ANP and hypoxia may play an important role in the proliferation and migration of PASMC, which may be of importance for hypoxic pulmonary vascular remodeling