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目的Ｗ基因是利用周围神经髓鞘蛋白ＰＭＰ－２２基因的编码区５′－及３′－序列为引物，藉ＰＣＲ方法自人脑胶质瘤ｃＤＮＡ文库中扩增的一个基因片段，本实验研究Ｗ基因组织表达的特异性。方法搜集不同组织来源的肿瘤及正常脑组织标本，行ＲＴ－ＰＣＲ及ＲＮＡ斑点杂交检测。结果在非神经组织来源的肿瘤及正常脑组织中未检出Ｗ基因表达，在一例星形胶质瘤Ⅲ级组织可检测到Ｗ基因表达。ＲＴ－ＰＣＲ及ＲＮＡ斑点杂交结果一致。结论提示Ｗ基因表达可能具有组织特异性。
Objective W gene is a gene fragment amplified from the cDNA library of human glioma by PCR using the 5’-and 3’-sequences of the coding region of PMP-22 gene as a primer. The present study Specificity of W gene expression. Methods Tumors and normal brain tissue samples from different tissues were collected and detected by RT-PCR and RNA dot blot. Results W gene expression was not found in tumors and normal brain tissues of non-nerve tissue origin. W gene expression was detected in grade III astrocytoma. RT-PCR and RNA dot blot hybridization results. Conclusions suggest that W gene expression may be tissue-specific.