论文部分内容阅读
目的规范小鼠超负荷哺乳模型,建立有效的促进乳汁分泌功能评价方法。方法以怀孕小鼠为研究对象,记录初产与第2胎孕鼠产仔量;2胎孕鼠分娩后,以窝(母鼠及其仔鼠)为基本单位,记录哺乳前后仔鼠体重改变,仔鼠存活情况,仔鼠和母鼠体重变化。结果初产孕鼠的产仔量(3~15只/窝)中位数为10只/窝,第二胎孕鼠产子量(5~19只/窝)为14只/窝。试验结束时,14、16只/窝组达到要求仔鼠量的窝数比例分别为28.6%、11.1%;16只/窝组平均仔鼠生存率为(77.1±19.5)%,与其他组比较差异有统计学意义(P<0.05);与其他组比较,8只/窝组母鼠体重在哺乳第3、6、9、18、21 d差异有统计学意义(P<0.05);不同哺乳时间(6、9、12、15、18、21 d)段,14、12只/窝组仔鼠平均增重均明显降低(P<0.05);12、10只/窝组母鼠每3d累积泌乳量在哺乳第11、14、17 d明显减少(P<0.05)。结论保健食品促进乳汁分泌的功能评价以第二胎孕鼠为试验对象较好,超负荷哺乳模型中母鼠的带仔量可选择12只/窝。
OBJECTIVE: To standardize the model of mice overloaded with lactation and to establish an effective evaluation method to promote milk secretion. Methods The pregnant mice were taken as the research object and the birth weight of primiparous and second pregnant fetus was recorded. After birth, the pregnant rats of 2 and 3 weeks old were sacrificed and their body weight was recorded , Offspring survival, offspring and maternal body weight changes. Results The median of litter size (3 ~ 15 / litter) of primiparous pregnant rats was 10 / litter and that of second litter pregnant rats was 14 / litter. At the end of the experiment, the number of litters reached to 14.6 / litter group was 28.6% and 11.1%, respectively. The average survival rate of 16 pups / litter group was (77.1 ± 19.5)%, compared with other groups (P <0.05). Compared with other groups, the body weight of maternal rats in 8 / litter groups was significantly different at 3, 6, 9, 18 and 21 days (P <0.05) At 6, 9, 12, 15, 18 and 21 days, the average weight gain of 14 and 12 litters / litter group were significantly decreased (P <0.05) The amount of lactation was significantly decreased on the 11th, 14th and 17th day after lactation (P <0.05). Conclusion The functional evaluation of health food to promote the secretion of milk to the second-fetus pregnant rats as the test object is better, overloaded breast-feeding model of the mother with the amount of piglets can choose 12 / nest.