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系统研究了Ｂｏｔｚｉｎｇｅｒ复合体（Ｂｏｔ．Ｃ）在呼吸节律调控中的作用。发现：（１）微电刺激Ｂｏｔ．Ｃ导致双相吸气抑制，潜伏期分析表明分别由单突触和多突触联系中介。（２）脊髓膈神经核局部微量注射ＧＡＢＡ受体阻断剂印防己毒素可以部分阻断Ｂｏｔ．Ｃ吸气抑制作用，提示ＧＡＢＡ参与中介Ｂｏｔ．Ｃ的吸气抑制作用。（３）双侧Ｂｏｔ．Ｃ微量注射ＧＡＢＡ导致呼吸节律消失，膈神经呈低幅度紧张放电，表明Ｂｏｔ．Ｃ在呼气相形成中起关键性作用。（４）ＨＲＰ逆行标记及逆行兴奋研究证明，Ｂｏｔ．Ｃ广泛接受脑干呼吸相关结构传入投射。结果表明Ｂｏｔ．Ｃ参与吸气时程和幅度的控制并在呼气相形成中起关键作用。
The role of Botzinger complex (Bot.C) in the regulation of respiratory rhythm was systematically investigated. It is found that: (1) Microelectrical stimulation Bot. C leads to biphasic inspiratory depression, and latency analysis shows intermediates linked by single synapses and multiple synapses, respectively. (2) Local injection of GABA receptor blocker picrotoxin into the phrenic nerve of the spinal cord partially blocked Bot. C inhalation inhibition, suggesting that GABA involved in the intermediary Bot. C inhalation inhibition. (3) bilateral Bot. C microinjection of GABA led to the disappearance of respiratory rhythm, phrenic nerve showed low amplitude tension discharge, indicating Bot. C plays a key role in the formation of expiratory phase. (4) HRP retrograde labeling and retrograde excitability studies have shown that Bot. C widely accepted brainstem respiration-related structures incoming projection. The results show that Bot. C participates in the control of inspiratory duration and amplitude and plays a key role in the formation of expiratory phase.