本工作研究水稻幼穗发育的不同时期,在离体培养过程中去分化和再分化能力的差异。实验主要结果如下: 1.随幼穗发育过程的进行,其愈伤组织的诱导率与绿苗分化率呈现出低→高→低的变化,有一个高峰点。当花粉母细胞进入减数分裂期则完全丧失了分化绿苗的能力。 2.与花药培养不同,材料差异对诱导率与分化率影响小,而幼穗发育时期的不同则起着主要作用。 3.处予Ⅱ、Ⅲ、Ⅳ期的幼穗同时具有向去分化和分化两个方向发展的趋势。 4.也会出现白化苗,但频率很低,仅占4%左右。 This work is to study the differences of dedifferentiation and re-differentiation ability in vitro during different stages of panicle development in rice. The main results of the experiment are as follows: 1. With the development of young panicles, the callus induction rate and green plantlet differentiation rate showed a low → high → low change, with a peak point. When pollen mother cells into meiosis, the ability to differentiate green seedlings is completely lost. 2. Different from the anther culture, the difference of materials has little effect on the induction rate and differentiation rate, while the difference of panicle development plays a major role. At the same time, young spikes of stage II, III and IV have the trend of dedifferentiation and differentiation. 4. There will be albino seedlings, but the frequency is very low, only about 4%.