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目的观察转化性生长因子β(TGF-β)对肝癌细胞的促进作用。方法 BUF大白鼠在接种7316A肝癌细胞的同时应用人γTGF-β_1;治疗,并于接种后5～20天测量肿瘤体积;γTGF-β_1还分别加入混合淋巴细胞培养(MLR)及7316A肝癌细胞培养中。结果大白鼠经TGF-β治疗组的肿瘤体积显著大于讨照组(P<0.01);TGF-β明显抑制T淋巴细胞的MLR反应(P<0.05),但对了316A肝癌细胞的增殖反应无明显抑制作用。结论由于TGF-β是肝再生过程产生的肝细胞再生抑制因子,提示肝硬化患者肝癌的发生率明显增高,可能与TGF-β抑制了免疫活性细胞的增殖,造成有利于肿瘤细胞生长的生物环境有关。
Objective To observe the promoting effect of transforming growth factor β (TGF-β) on hepatocellular carcinoma cells. Methods BUF rats were treated with human γTGF-β_1 while inoculated with 7316A hepatoma cells. Tumor volume was measured 5 to 20 days after inoculation. γTGF-β_1 was also added to mixed lymphocyte culture (MLR) and 7316A hepatoma cell cultures, respectively. . Results The tumor volume of rats treated with TGF-β was significantly greater than that of the treated group (P<0.01); TGF-β significantly inhibited the TLR lymphocyte MLR response (P<0.05), but the proliferation response of 316A hepatoma cells was not Obvious inhibitory effect. Conclusions TGF-β is a hepatocyte regeneration inhibitory factor produced during liver regeneration, suggesting that the incidence of hepatocellular carcinoma in patients with cirrhosis is significantly higher, and may inhibit the proliferation of immunocompetent cells with TGF-β, resulting in a biological environment conducive to the growth of tumor cells. related.