目的探讨稳肾合剂(山茱萸、薏苡仁、大黄等)的质量控制方法。方法采用TLC法对薏苡仁、大黄和黄连进行定性鉴别;采用HPLC法测定马钱苷含量。结果建立的TLC方法斑点清晰,分离度好。含量测定结果显示,马钱苷在0.333~10.67μg(r=0.9999)范围内线性关系良好,平均回收率大于95%。结论该方法可靠准确,专属性好,可以用于稳肾合剂的质量控制。 Objective To investigate the quality control method of Weishen Mixture (Cornus officinalis, Coix seed, Rhubarb). Methods TLC method was used to identify Coix seed, rhubarb and berberine. The content of loganin was determined by HPLC. Results TLC method established clear spots, good resolution. The content of loganin showed good linearity in the range of 0.333 ~ 10.67μg (r = 0.9999) with the average recovery of more than 95%. Conclusion The method is reliable and accurate, and has good specificity. It can be used for the quality control of Stomach Deficiency and Kidney Stomach Mixture.