采用体外培养方法,观察集落刺激因子-1(CSF-1)对小鼠骨髓细胞和腹腔巨噬细胞的影响。结果发现在新鲜分离的骨髓细胞中,巨噬细胞只占4.5％,在含有CSF-1(100u/ml)的培养体系中,经过7～8d培养巨噬细胞的比例达98％。这些细胞能粘附于塑料表面,呈典型的巨噬细胞形态。研究结果还表明CSF-1对小鼠腹腔巨噬细胞的吞噬、杀菌能力和抗病毒(VSV)能力都有明显的增强作用。 The effects of CSF-1 on bone marrow cells and peritoneal macrophages were observed by in vitro culture. As a result, macrophages accounted for only 4.5% of freshly isolated myeloid cells, and 98% of macrophages were cultured for 7 to 8 days in a culture system containing CSF-1 (100u / ml). These cells can adhere to the plastic surface, the typical macrophage morphology. The results also show that CSF-1 can significantly enhance phagocytosis, bactericidal and anti-virus (VSV) ability of mouse peritoneal macrophages.