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作者观察了组织块法和酶消化法以及DMEM和RPMI1640二种常用培养液在人牙髓细胞体外培养中的效果。结果表明，组织块法培养出的牙髓细胞为单一的成纤维细胞样细胞，用DMEM时，其传代成功率明显高于用RPMI1604；胰蛋白酶消化法、胰蛋白酶和胶原酶联用可培养出成纤维细胞样细胞，少量的校形细胞和内皮样细胞，但细胞数量较少，生长缓慢，达不到传代要求。DMEM在培养板和组织培养瓶中均较适合牙髓细胞的生长，而RPMI1640效果不理想。提示组织块法和DMEM适合于人牙髓细胞的体外培养。
The authors observed the tissue block method and enzyme digestion and DMEM and RPMI1640 two kinds of commonly used culture medium in human dental pulp cells in vitro culture effect. The results showed that the tissue culture of dental pulp cells as a single fibroblast-like cells, with DMEM, the success rate was significantly higher than with RPMI1604; trypsin digestion, trypsin and collagenase can be cultured Fibroblast-like cells, a small amount of cells and endothelium-like cells, but the number of cells is small, slow growth, reaching less than the passage requirements. DMEM in the culture plate and tissue culture flask are more suitable for the growth of dental pulp cells, while the effect of RPMI1640 is not satisfactory. Tissue block method and DMEM suggest that human dental pulp cells in vitro culture.