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自应用溴脱氧尿核苷(BUdR)替换哺乳动物染色体,显示姐妹染色单体交换(SCEs)简单染色技术以来,对各种诱变剂引起细胞遗传损伤效应,已有十多篇报告,看到哺乳动物细胞在体内外受许多化学制剂作用时,SCEs率增加。由于SCE对射线敏感,认为应用本法作分裂实验是一种快速方法。作者以~(60)Coγ线150伦或300伦照射人的Go期淋巴细胞,对SCE率进行了观察。本文采用两名健康人肝素抗凝血,自然沉降1小时,取白细胞层血浆0.8ml,放入8ml199培养液内,加抗菌素和1.2ml自身血浆。每分血分三并培养,一并对照,其余两并于37℃以150或300R~(60)Coγ线进行照射,剂量率为50.2伦/分,然后立即加PHA0.1ml和BUdR10μg/ml,37℃培养,68小时加秋水仙素10μg/ml,72小时收获细胞,用改进的FPG法染色,分析SCE。
Since the replacement of mammalian chromosomes with bromodeoxyuridine (BUdR) and the simple staining of sister chromatid exchange (SCEs) techniques, a variety of mutagens have been shown to cause cytogenetic damage effects, which have been reported for more than ten years The rate of SCEs increases when mammalian cells are subjected to many chemical agents both in vitro and in vivo. Due to the sensitivity of SCE to radiation, it is considered a quick method to use this method for the experiment of splitting. The authors used a ~ (60) Coγ line 150 Lun or 300 Lun irradiated human Go lymphocytes, the SCE rate was observed. In this paper, two healthy human anticoagulant heparin, natural sedimentation for 1 hour, take the plasma leukocyte layer 0.8ml, into 8ml199 culture medium, add antibiotics and 1.2ml of plasma. The mice were divided into three groups and divided into three groups. The remaining two were irradiated with 150 or 300R ~ (60) Coγ line at 37 ℃. The dose rate was 50.2 LUN / min, then PHA0.1ml and BUdR10μg / ml were added immediately, The cells were cultured at 37 ℃, colchicine (10μg / ml) was added for 68 hours, and the cells were harvested at 72 hours. The cells were stained with improved FPG method to analyze SCE.