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目的观察冷冻消融后残存肿瘤组织中微血管密度(MVD)及血管内皮生长因子(VEGF)的表达情况,探讨肿瘤血管生成的机制及意义。方法建立肺腺癌A549裸鼠皮下移植瘤模型18只,待肿瘤最大直径达1 cm随机分为3组:对照组、顺铂(DDP)组、冷冻消融组。冷冻消融后第21天处死裸鼠,取出肿瘤组织,免疫组织化学SP法检测肿瘤组织MVD及VEGF表达水平。结果对照组、DDP组、冷冻消融组的肿瘤体积分别为(1.48±0.14)cm3、(1.03±0.12)cm3、(0.99±0.06)cm3(P<0.01);MVD值分别为(21.10±0.86)、(24.70±0.72)、(29.17±0.96)(P<0.01);VEGF阳性表达水平分别为(36.17±1.72)%、(39.00±1.79)%、(50.83±2.14)%(P<0.01)。MVD与VEGF阳性表达之间呈正相关关系(r=0.928,P<0.01)。结论冷冻消融能够有效地控制肿瘤生长,但残存肿瘤内新生血管明显增多,VEGF的高表达对残存肿瘤血管生成起到了重要作用。
Objective To observe the expression of microvessel density (MVD) and vascular endothelial growth factor (VEGF) in residual tumor tissue after cryoablation and explore the mechanism and significance of tumor angiogenesis. Methods 18 nude mice were randomly divided into 3 groups: control group, cisplatin (DDP) group and cryoablation group. On day 21 after cryoablation, the nude mice were sacrificed and the tumor tissues were removed. The expression of MVD and VEGF in the tumor tissues were detected by immunohistochemical SP method. Results The tumor volume of control group, DDP group and cryoablation group were (1.48 ± 0.14) cm3, (1.03 ± 0.12) cm3 and (0.99 ± 0.06) cm3 respectively (P <0.01) .The MVD values were (21.10 ± 0.86) , (24.70 ± 0.72) and (29.17 ± 0.96), respectively (P <0.01). The positive expression of VEGF was (36.17 ± 1.72)%, (39.00 ± 1.79)% and (50.83 ± 2.14)%, respectively. There was a positive correlation between MVD and VEGF expression (r = 0.928, P <0.01). Conclusion Cryoablation can effectively control tumor growth, but the number of neovascularization in the residual tumor is significantly increased. The high expression of VEGF plays an important role in the angiogenesis of residual tumor.