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目的 :通过研究糖皮质激素受体 β对糖皮质激素受体α功能的影响 ,探讨糖皮质激素受体 β可能存在的生物学意义。方法 :( 1 )用瞬时转染技术将ＧＲβ和报告基因ｃａｔ共转染ＨＯＳ - 860 3细胞 ,激素处理后 ,研究ＧＲβ对糖皮质激素诱导ＧＲα外源性靶基因ｃａｔ表达的影响 ;(
Objective: To explore the biological significance of glucocorticoid receptor β by studying the effect of glucocorticoid receptor β on the glucocorticoid receptor α function. Methods: (1) Transient transfection technique was used to co - transfect GRβ and reporter gene cat into HOS - 8603 cells. After treatment with hormones, the effect of GRβ on cat expression of exogenous GRα gene induced by glucocorticoid was studied.