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目的 :血管平滑肌细胞 (ＶＳＭＣｓ)增殖与迁移在动脉粥样硬化 (ＡＳ)的早期、中期或ＡＳ进展期或血管事件的发病机制中起着关键性作用。方法 :采用改良的Ｂｏｙｄｅｎ小室法检测ＶＳＭＣｓ的迁移效应 ;以ＭＴＴ法、[3Ｈ]-Ｔｄｒ掺入法、[3Ｈ]-脯氨酸标记法和ＶＳＭＣｓ计数评
OBJECTIVE: Proliferation and migration of vascular smooth muscle cells (VSMCs) play a key role in the pathogenesis of early, mid-stage or AS progression of atherosclerosis (AS) or vascular events. Methods: The migration of VSMCs was detected by a modified Boyden chamber method. MTT assay, [3H] -Tdr incorporation, [3H] -proline labeling and VSMCs counting