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目的 研讨非特异性酯酶 (Non -specificesterase ,NSE)活力在抗DDVP和敏感品系淡色库蚊不同发育期的变化 ,为应用生物化学方法检测蚊虫抗药性及科学合理地进行蚊虫化学防治提供依据。方法 以 β -乙酸萘酯为底物 ,坚固蓝B盐为显色剂 ,用微量滴定板法测定单个蚊虫NSE活力。结果 敏感品系和抗DDVP品系淡色库蚊NSE活力水平在不同发育期有变化 ,Ⅰ～Ⅲ龄幼虫较低 ,Ⅳ龄幼虫最高 ,蛹期略下降 ,成虫期较高。各发育期抗DDVP品系NSE活力均高于敏感品系 ,Ⅳ龄幼虫和成虫期二者差别明显。结论 测定NSE检测蚊虫抗药性可选择Ⅳ龄幼虫或 3日龄未吸血雌成蚊 ;蚊虫化学防治中应选择低龄幼虫期施药。
Objective To investigate the changes of non-specific esterase (NSE) activity at different developmental stages of Culex pipiens quinquefasciatus, an anti-DDVP and susceptible strain, and provide a basis for biochemical detection of mosquito resistance and scientific and rational chemical control of mosquitoes. Methods β - naphthyl acetate as substrate and solid blue B as color reagent were used to measure the NSE activity of individual mosquitoes by microtiter plate method. Results The NSE activity of Culex pipiens pallens in susceptible and anti-DDVP strains varied at different developmental stages. The larvae of stage I to III were lower, the larva of stage IV were the highest, the larvae of stage IV were slightly lower and the stage of larvae was higher. The activities of NSE in anti-DDVP strains at all developmental stages were higher than those in susceptible strains, and the difference between the fourth-instar larvae and the adult was significant. Conclusion NSE can detect the resistance of mosquitoes to choose the fourth instar larvae or 3-day-old non-vampire adult mosquitoes; mosquito chemical control should be selected low-instar larvae application.