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我们之前已经研发了一种生物工程化的左旋聚乳酸(PLLA)前交叉韧带(ACL)基质,该基质在植入兔模型之前被接种了原代ACL细胞,表现出比基质单独作用时更强的愈合能力.这表明改善细胞在基质上的黏附性可能有益于生物工程化的ACL基质的愈合反应和长期性能.一种与即时诊疗干细胞疗法相结合的再生工程方法是,增强基质的表面特性来支持细胞黏附和生长.在本文中,我们研究了通过纤连蛋白的物理吸附和空气等离子体处理来增强PLLA编织超细纤维基质的细胞黏附性能.我们在三个时间点和三个纤连蛋白浓度下评估了纤连蛋白在基质上的动力学和结合效率.在25 μg·mL-1纤连蛋白溶液中孵育120 min,可实现基质的最大结合效率和细胞黏附性.在纤连蛋白吸附之前将基质置于空气等离子体中处理5 min,可显著增强接种24 h后兔骨髓间充质干细胞(R-BMMSC)的黏附性.最后,监测细胞增殖长达21 d,将基质用空气等离子体处理后,发现纤连蛋白的吸附性导致细胞数量增加.这些发现表明,空气等离子体处理和纤连蛋白吸附可以增强PLLA编织超细纤维基质的细胞黏附性,可改善基质在即时诊疗干细胞疗法中的临床疗效.“,”We have previously developed a poly(L-lactic) acid (PLLA) bioengineered anterior cruciate ligament (ACL) matrix that has demonstrated enhanced healing when seeded with primary ACL cells prior to implanta-tion in a rabbit model, as compared with the matrix alone. This suggests that improving cell adhesion on the matrix may beneficially affect the healing response and long-term performance of the bioengineered ACL matrix. One regenerative engineering approach involves enhancing the surface properties of the matrix to support cell adhesion and growth in combination with point-of-care stem cell therapy. Herein, we studied the cell adhesion properties of PLLA braided microfiber matrices enhanced through the physical adsorption of fibronectin and air plasma treatment. We evaluated the kinetics and binding efficiency of fibronectin onto matrices at three time points and three fibronectin concentrations. Incubating the matrix for 120 min in a solution of 25 μg?mL 1 fibronectin achieved the greatest binding efficiency to the matrix and cellular adhesion. Exposing the matrices to air plasma treatment for 5 min before fibronectin adsorption significantly enhanced the cell adhesion of rabbit bone marrow-derived mesenchymal stem cells (R-BMMSCs) 24 h post cell seeding. Finally, cellular proliferation was monitored for up to 21 d, the matrices were exposed to air plasma treatment, and fibronectin adsorption was found to result in enhanced cell number. These findings suggest that exposure to air plasma treatment and fibronectin adsorption enhances the cellular adhesion of PLLA braided microfiber matrices and may improve the clinical efficacy of the matrix in combination with point-of-care stem cell therapies.