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Background Taxifolin(Tax) is an essential natural antioxidant. Multiple studies have shown that Tax can protect cardiomyocytes from ischemia-reperfusion injury. However, the underlying mechanism is still unclear.Methods H9C2 cells were randomly divided into control, H_2O_2 group, Tax pretreatment group(Tax + H_2O_2);Tax effect group. Cell activity was detected by CCK-8 and the intracellular structure was observed by transmission electron microscopy. Autophagy was determine by Western blotting analysis of Beclin-1, Bcl-2 and PKC.Results Tax pretreatment significantly increased anti-apoptotic protein Bcl-2 and autophagy protein Beclin-1.Expression of PKC was inhibited by Tax. Conclusions Tax pretreatment could protect H9 C2 cells against H_2O_2-induced damage through the Bcl-2 and autophagy pathways.
However, the underlying mechanism is still unclear. Methods H9C2 cells were randomly divided into control, H 2 O 2 group, Tax pretreatment group (Tax + H 2 O 2); Tax effect group. Cell activity was detected by CCK-8 and the intracellular structure was observed by transmission electron microscopy. Autophagy was determined by Western blotting analysis of Beclin-1, Bcl-2 and PKC. Results Tax pretreatment Significively increased anti-apoptotic protein Bcl-2 and autophagy protein Beclin-1. Expression of PKC was inhibited by Tax. Conclusions Tax pretreatment could protect H9 C2 cells against H 2 O 2-induced damage through the Bcl-2 and autophagy pathways.