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目的:观察普罗布考对大鼠颈动脉球囊损伤后狭窄的抑制作用,探索其可能机制。方法:随机将45只SD大鼠分为假手术组(n=15)、对照组(n=15)和治疗组(n=15),对照组及治疗组均用球囊损伤左颈总动脉,治疗组给予普罗布考灌胃。术后7、14、35 d处死大鼠,放免法检测大鼠血浆MDA,SOD水平;伊文思蓝染色观察血管内皮修复;PCNA染色检测细胞增殖;Image-Pro plus系统进行血管定量组织形态学分析。结果:普罗布考明显升高血浆SOD水平,降低MDA水平;伊文思蓝染色结果显示普罗布考抑制内膜增生同时并未影响血管内皮化;治疗后14 d,治疗组PCNA阳性细胞指数较对照组明显降低(n=15,P<0.01),内膜及中膜平滑肌细胞的抑制率为76%和65%,差异有统计学意义(P<0.05);治疗后35 d,与对照组比较,治疗组降低内膜/中膜面积比达62.63%。结论:普罗布考能升高血浆SOD水平、降低MDA水平,明显抗氧化,抑制血管平滑肌细胞异常增殖和新生内膜的形成,预防大鼠颈动脉球囊损伤后再狭窄。
Objective: To observe the inhibitory effect of probucol on the stenosis after carotid artery balloon injury in rats and explore its possible mechanism. Methods: Forty-five SD rats were randomly divided into sham operation group (n = 15), control group (n = 15) and treatment group (n = 15) The treatment group was fed probucol. The rats were killed at 7, 14 and 35 days after operation, and the levels of MDA and SOD in plasma were detected by radioimmunoassay; the vascular endothelial cells were repaired by Evans blue staining; the cell proliferation was detected by PCNA staining; . Results: Probucol significantly increased plasma SOD level and decreased MDA level. Evans blue staining showed that probucol inhibited endometrial hyperplasia and did not affect vascular endothelialization. On the 14th day after treatment, PCNA positive cells index (N = 15, P <0.01). The inhibitory rates of smooth muscle cells in intima and media were 76% and 65%, respectively, with statistical significance (P <0.05). After 35 days of treatment, compared with the control group , The treatment group decreased intima / media area ratio of 62.63%. CONCLUSION: Probucol can increase the level of plasma SOD, decrease the level of MDA, significantly inhibit the proliferation of vascular smooth muscle cells and the formation of neointima, and prevent the restenosis after carotid artery balloon injury in rats.