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目的:探讨ERK1/2和Survivin在食管癌中的表达和二者之间的相关性。方法:应用免疫组化法检测40例食管癌和20例正常食管标本中ERK1/2和Survivin的表达。Westernblot法检测ERK1/2和Survivin在食管癌中的表达以及PD98059对食管癌Eca109细胞株ERK1/2、Survivin表达的影响。免疫共沉淀法检测ERK1/2和Survivin在食管癌Eca109细胞株中的相互作用。结果:ERK1/2和Survivin在食管癌中表达的阳性率分别87.5%和90.0%,在正常食管组织中表达的阳性率为40.0%和5.0%。Westen blot显示ERK1/2和Survivin在食管癌癌组织中的表达高于癌旁组织中的表达。免疫共沉淀结果提示ERK1/2和Survivin在食管癌中的表达存在相互作用。PD98059下调Survivin的表达。结论:ERK1/2和Survivin在食管癌中高表达,在食管癌Eca109细胞中ERK1/2和Survivin表达有相关性,抑制ERK1/2的表达能下调Survivin的表达。在食管癌中Survivin的表达可能依赖于ERK1/2。
OBJECTIVE: To investigate the expression of ERK1 / 2 and Survivin in esophageal cancer and the correlation between them. Methods: The expressions of ERK1 / 2 and Survivin in 40 cases of esophageal carcinoma and 20 cases of normal esophagus were detected by immunohistochemistry. Western blot was used to detect the expression of ERK1 / 2 and Survivin in esophageal carcinoma and the effect of PD98059 on the expression of ERK1 / 2 and Survivin in esophageal carcinoma cell line Eca109. Immunoprecipitation assay was used to detect the interaction between ERK1 / 2 and Survivin in esophageal carcinoma cell line Eca109. Results: The positive rates of ERK1 / 2 and Survivin expression in esophageal carcinoma were 87.5% and 90.0%, respectively. The positive rates of ERK1 / 2 and Survivin expression in normal esophageal tissues were 40.0% and 5.0% respectively. Westen blot showed that the expression of ERK1 / 2 and Survivin in esophageal cancer tissues was higher than that in paracancerous tissues. Immunoprecipitation results suggest that ERK1 / 2 and Survivin expression in esophageal cancer there is interaction. PD98059 downregulates Survivin expression. CONCLUSIONS: ERK1 / 2 and Survivin are overexpressed in esophageal cancer, and there is a correlation between ERK1 / 2 and Survivin expression in esophageal cancer Eca109 cells. Inhibition of ERK1 / 2 down-regulates Survivin expression. Survivin expression in esophageal cancer may depend on ERK1 / 2.