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观察大黄素对胰岛素抵抗大鼠脂肪组织11β-HSDl表达的影响,探讨其改善胰岛素抵抗的可能机制。采用30只雄性Wistar大鼠作为研究对象,随机分为对照组、模型组和给药组,每组10只。模型建立通过高脂饲料喂养结合地塞米松刺激的方式。12周后造模成功,给药组予大黄素200 mg·kg-1灌胃。给药4周后,进行胰岛素耐量试验,检测血糖、血脂、血液胰岛素及皮质酮水平,计算肝指数、内脏肥胖指数及HOMA-IR指数和11β-HSD1基因及蛋白表达情况。结果表明,与模型组比较,给药组胰岛素抵抗状态显著改善,血糖、胰岛素及血脂水平明显降低,肝指数及内脏肥胖指数也明显下降,同时内脏脂肪组织11β-HSD1基因及蛋白表达明显下调。可见大黄素可显著改善胰岛素抵抗大鼠的糖脂代谢及胰岛素敏感性,其作用机制可能与抑制11β-HSD1基因及蛋白表达有关。
To observe the effect of emodin on the expression of 11β-HSDl in adipose tissue of insulin resistance rats and to explore its possible mechanism of improving insulin resistance. Thirty male Wistar rats were randomly divided into control group, model group and administration group, with 10 rats in each group. The model establishes the way of feeding with dexamethasone stimulated by high fat diet. After 12 weeks, the model was successfully established. The emodin group was given 200 mg · kg-1 orally. After 4 weeks of administration, insulin resistance test, blood glucose, blood lipids, blood insulin and corticosterone levels were measured. Liver index, visceral obesity index and HOMA-IR index and 11β-HSD1 gene and protein expression were calculated. The results showed that compared with the model group, the state of insulin resistance in the administration group was significantly improved, blood glucose, insulin and blood lipid levels were significantly decreased, liver index and visceral obesity index were significantly decreased, while 11β-HSD1 gene and protein expression in visceral adipose tissue was significantly decreased. It can be seen that emodin can significantly improve glucose and lipid metabolism and insulin sensitivity in insulin resistance rats, which may be related to the inhibition of 11β-HSD1 gene and protein expression.