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目的研究评价大黄醇提液的抗乙肝病毒作用。方法体内实验采用先天感染鸭乙肝模型,检测血清DHBV-DNA动态变化,体外实验采用2.2.15细胞为模型,检测用药后对细胞培养上清中HBsAg和HBeAg的抑制作用。结果在体内抗乙肝病毒实验中,大黄醇提液20g/kg和10g/kg剂量组于给药5d(T5)、10d(T10)和停药3d(P3)时体内均有显著抑制DHBV-DNA的作用。体外实验显示:大黄醇提液对2.2.15细胞分泌HBsAg的治疗指数为16.96,对分泌HBeAg的治疗指数为12.06。结论大黄醇提液在体内外实验中均具有明显的抗乙肝病毒作用。
Objective To evaluate the anti-hepatitis B virus effect of Rhubarb alcohol extract. Methods In vivo experiments using congenital infection of duck hepatitis B model to detect the dynamic changes of serum DHBV-DNA, in vitro experiments using 2.2.15 cells as a model to detect the effect of drugs on the cell culture supernatant in the inhibition of HBsAg and HBeAg. Results In vivo anti-HBV test, the Rhubarb alcohol extracts 20g/kg and 10g/kg dose group significantly inhibited DHBV-DNA in vivo after 5d (T5), 10d (T10) and 3d (P3) withdrawal. The role. In vitro experiments showed that the therapeutic index of rhubarb alcohol extract on HBsAg secretion in 2.2.15 cells was 16.96, and the therapeutic index for HBeAg secretion was 12.06. Conclusion Rhubarb alcohol extract has obvious anti-hepatitis B virus effect both in vitro and in vivo.