目的研究温莪术内生真菌的种群组成及其代谢产物抗菌活性。方法采用内生菌常规分离法对健康温莪术植株体内的内生真菌进行了分离鉴定。以大肠杆菌、金黄色葡萄球菌、链球菌、沙门氏菌、巴氏杆菌为测试菌种,对温莪术根、茎、叶中分离的56株内生真菌进行抗菌活性筛选。结果经初步鉴定,这些内生真菌为5目、6科、24属;抑菌实验表明,56株内生真菌中有39株对5种测试菌均有不同程度的抑制作用。结论温莪术植物含有丰富多样的内生真菌,且多数内生真菌都表现为较高的抑菌活性。 Objective To study the population composition and the antimicrobial activity of endophytic fungi of Curcuma curcuma. Methods Endophytic fungi were isolated and identified by normal endophyte isolation method. The antibacterial activity of 56 endophytic fungi isolated from roots, stems and leaves of Rhizoma Curcumae was screened by using Escherichia coli, Staphylococcus aureus, Streptococcus, Salmonella and Pasteurella as tested strains. Results The results showed that these endophytic fungi were 5 orders, 6 families and 24 genera. The antibacterial experiment showed that 39 of the 56 endophytic fungi had different degrees of inhibitory effect on the five tested bacteria. Conclusion The genus Curcuma contains rich and diverse endophytic fungi, and most of the endophytic fungi showed high antibacterial activity.