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目的:研究微波辅助萃取马铃薯叶中茄尼醇的最佳工艺条件。方法:以95%乙醇为溶媒,高效液相色谱法测定提取液中茄尼醇的含量,采用L16(44)正交设计法考察料液比、萃取时间、微波辐照功率和萃取温度四因素对茄尼醇提取率和浸膏得率的影响。结果:4个因素对马铃薯叶中茄尼醇提取工艺的影响顺序依次为萃取温度、料液比、微波辐照功率、萃取时间,其中萃取温度对提取工艺有显著性影响(P<0.05)。结论:用95%的乙醇作溶媒微波辅助萃取马铃薯叶中茄尼醇的最佳工艺条件为料液比1∶8(g.mL-1),提取时间20min,提取温度65℃,微波功率450W。在此条件下茄尼醇的提取率为94.19%。
Objective: To study the optimum conditions of solanesol extraction by microwave in potato leaves. Methods: The content of solanesol in the extract was determined by high performance liquid chromatography (HPLC) with 95% ethanol as solvent. The orthogonal design of L16 (44) was used to study the factors such as the ratio of material and liquid, the extraction time, the power of microwave irradiation and the extraction temperature Effect of solanesol extraction rate and extract yield. Results: The four factors influencing the extraction of solanesol from potato leaves were as follows: extraction temperature, solid-liquid ratio, microwave irradiation power and extraction time. The extraction temperature had significant influence on the extraction process (P <0.05). CONCLUSION: The optimal conditions of solanesol extraction from potato leaves with 95% ethanol as solvent are: extraction ratio of 1: 8 (g.mL-1), extraction time of 20 min, extraction temperature of 65 ℃, microwave power of 450W . In this condition solanesol extraction rate was 94.19%.