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千粒重和垩白粒率是水稻重要的产量和品质性状。本研究以粳稻Sasanishiki为受体、籼稻品种Habataki为供体构建的染色体片段渗入系群体(Introgression Lines,ILs)为材料,在两个不同的环境下进行了千粒重和垩白粒率相关性分析和QTL定位。相关分析表明,在两个不同的环境下,群体千粒重和垩白粒率之间相关性不显著。两个环境共检测到9个稻谷千粒重QTL、5个糙米千粒重QTL和6个垩白粒率QTL,分布在水稻的10条染色体上。在两个不同的环境下重复检测到其中的5个QTL,即影响稻谷千粒重的qPTGW3、qPTGW8.2和qPTGW11.1及控制垩白粒率qPGWC1.1和qPGWC1.2。其所对应染色体片段渗入系的相应性状与背景亲本Sasanishiki的表现型差异在连续两个环境中均达到显著(P<0.05)或极显著(P<0.01)水平。这些材料为优良粒重和垩白粒率QTL的克隆及水稻产量和外观品质的标记辅助选择(MAS)育种利用提供了基础。
Grain weight and chalky grain rate are important yield and quality traits in rice. In this study, we analyzed the correlation between 1000-grain weight and chalky grain rate in two different environments by using japanica Sasanishiki as receptor, Habataki indica variety Introgression Lines (ILs) as donor. QTL mapping. Correlation analysis showed that in two different environments, there was no significant correlation between 1000-grain weight and chalky grain rate. Two QTLs for 1000-grain weight, 5 QTLs for 1000-grain weight of brown rice and 6 QTLs for chalkiness were detected in the two environments, which were distributed on 10 chromosomes in rice. Five of these QTLs were repeatedly detected in two different environments, qPTGW3, qPTGW8.2 and qPTGW11.1, which affect 1000-grain weight, and control of chalky grain rate qPGWC1.1 and qPGWC1.2. The corresponding phenotypes of the corresponding chromosome introgression lines and the phenotypic differences of background parents Sasanishiki reached significant (P <0.05) or very significant (P <0.01) levels in two consecutive environments. These materials provided the basis for the cloning of QTLs for fine grain weight and chalky grain rate and the use of marker-assisted selection (MAS) for rice yield and appearance quality.