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目的通过研究Dicer蛋白表达水平与口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)临床病理特征的关系探讨其在OSCC发展过程中的作用。方法利用免疫组化法对76例OSCC、20例白斑、10例癌旁非肿瘤性口腔上皮(normal adjacent to the tumor,NAT)样本的Dicer酶表达进行评估研究。利用Image-Pro Plus 6.0图像分析系统计算平均光密度值(mean optical density,MOD),SPSS 14.0软件系统分析Dicer蛋白MOD、阳性细胞百分比(percentage of positive cells,PP)与临床病理参数的关系。结果 OSCC、白斑组织、NAT的Dicer蛋白MOD、PP依次递减,三者间差异有统计学意义(P<0.05)。Dicer过度表达与肿瘤分期呈正相关关系(P<0.01),与淋巴结转移存在正相关关系(P<0.05),与年龄、性别、分化程度无相关关系。结论 Dicer蛋白表达上调可能通过影响OSCC的生长以及淋巴转移而参与OSCC的发生发展。
Objective To investigate the role of Dicer protein in the development of OSCC by investigating the relationship between the expression of Dicer protein and clinicopathological features of oral squamous cell carcinoma (OSCC). Methods Immunohistochemical method was used to evaluate the expression of Dicer in 76 cases of OSCC, 20 cases of leukoplakia and 10 cases of normal adjacent to the tumor (NAT). The mean optical density (MOD) was calculated by Image-Pro Plus 6.0 image analysis system. The relationship between Dicer protein MOD, percentage of positive cells (PP) and clinicopathological parameters was analyzed by SPSS 14.0 software. Results The Dicer protein MOD, PP of OSCC, leukoplakia and NAT decreased in descending order, with significant difference (P <0.05). There was a positive correlation between Dicer overexpression and tumor stage (P <0.01), and lymph node metastasis (P <0.05), but not with age, sex and differentiation. Conclusion Upregulation of Dicer protein may be involved in the development of OSCC by affecting the growth and lymph node metastasis of OSCC.