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用原始栽培种 Solanum phureja作为抗源构建了一个二倍体马铃薯作图群体 ,并且用于进行群分法 (bulked segregant analysis,BSA)分析 ,以筛选检定马铃薯青枯病抗性的 RAPD连锁标记。使用 30 0个随机引物进行 RAPD检测 ,发现引物 OPG0 9可在抗感性 DNA池之间产生 96 0 bp的稳定的相斥型多态性产物。进一步分析分离群体并得到与抗性相连锁的标记 OPG0 9960 。该标记已有效地应用于检测其它具相近遗传背景的二倍体群体的抗性
A diploid potato mapping population was constructed using the original cultivar Solanum phureja as a source of resistance and was used for bulked segregant analysis (BSA) analysis to screen RAPD markers for potato bacterial wilt resistance. Using 30 random primers for RAPD detection, the primer OPG0 9 was found to produce a stable 96O bp stable repulsion polymorphism product between anti-sense DNA pools. Further analysis of segregation populations and identification of marker OPG0 9960 linked to resistance. This marker has been effectively applied to detect the resistance of other diploid populations with similar genetic backgrounds