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目的观察过氧化氢与金黄色葡萄球菌接触后所产生的泡沫对杀菌效果的影响,寻求克服方法。方法采用悬液定量杀菌试验和载体浸泡定量杀菌试验方法,对过氧化氢液体与菌液混合产生的泡沫影响杀菌效果的因素进行观察。结果以4∶1比例的34 g/L过氧化氢消毒液与菌液混合后,即产生较明显的气泡,肉眼可见粘附在管壁,微量法1泡沫稍轻。常量法试验回收菌量≤2.0×107cfu/ml,作用30 min平均杀灭对数值3.04;微量法1同比例微量法试验回收菌量<2.0×107cfu/ml,平均杀灭对数值为3.20,而微量法2回收菌量≥2.0×107cfu/ml时平均杀灭对数值则达到5.36。载体定量杀菌试验中未发现产生泡沫现象,对金黄色葡萄球菌杀灭对数值均达到消毒合格要求。结论过氧化氢消毒液与金黄色葡萄球菌悬液经触酶分解产生的大量泡沫粘附管壁会影响其杀菌效果,采用微量实验和载体浸泡杀菌试验方法可以消除这种影响。
Objective To observe the effect of foam produced by the contact of hydrogen peroxide with Staphylococcus aureus on the bactericidal effect and to find a way to overcome it. Methods Quantitative bactericidal test of suspensions and quantitative bactericidal test of carrier soaking were used to observe the factors affecting the bactericidal effect of the foam produced by the mixture of hydrogen peroxide liquid and bacterial liquid. Results 4: 1 ratio of 34 g / L hydrogen peroxide disinfectant mixed with bacteria, that is more obvious bubbles, visible adhesion to the wall, trace method 1 foam slightly lighter. In the constant method test, the recovering bacteria amount was less than 2.0 × 107cfu / ml and the mean killing logarithm was 30 minutes after the 30 min reaction. Micropropagation method 1 showed that the average recovering bacteria number was 2.0 × 107cfu / ml and the average killing logarithm was 3.20 Trace method 2 recovery of bacteria ≥ 2.0 × 107cfu / ml when the average kill logarithm reached 5.36. Quantitative bactericidal test was not found in the foam phenomenon, the killing of Staphylococcus aureus to achieve the number of sterilization requirements. Conclusion The large amount of foam adhesion tube wall produced by hydrogen peroxide disinfectant and Staphylococcus aureus suspension can affect its bactericidal effect. This effect can be eliminated by micro-experiment and carrier immersion sterilization test.